Available online at CURCUMA CAESIA ROXB. AND IT’S MEDICINAL. USES: A REVIEW. Sonjit Das, ProdyutMondal and Md. Kamaruz Zaman*. PDF | On Apr 19, , Sonjit Das and others published CURCUMA CAESIA ROXB. AND IT’S MEDICINAL USES: A REVIEW. Black turmeric is known to have certain medicinal values. The curcumin content when I read certain papers, It was very low ~ %. Now I am wondering.
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With an ever increasing cause of cancer, it has been recommended to treat with conventional drugs, however because of the side effects caused by the conventional drugs, the research on medicinal plants has been intensified due to their less adverse and toxic effects.
The primary objective of the present study was to evaluate the protective effect of the medicinal plant Curcuma caesia Roxb. Also it was aimed to caeesia the protective effect of C. For antigenotoxic studies, animals were divided into eight groups and micronucleus assay was employed and for biochemical analysis serum sample was collected from the blood and SGOT, SGPT analysis was performed.
Also the biochemical analysis was performed from both the liver and kidney. The methanolic extract of Curcuma caesia Roxb. The increased level of serum SGPT and SGOT as well as peroxidation level in both liver and kidney due to treatment of cyclophosphamide was also found to be decreased with the pretreatment of the extract significantly as compared to the positive control groups.
There was decreased in curvuma level of endogenous antioxidant such as GSH and GR in the positive control group however decreased level of GSH and GR was found to be increased with the pretreatment of the methanolic extract of C. The present study suggested that the methanolic extract of C. It was also to have the protective effects against the liver and kidney.
So it could be provided as one of the herbal supplementation in chemoprevention of Casia to ameliorate the side effects of it. With an ever increasing cause of cancer due to diet, environment, and carcinogenic virus[ 12 ] conventional drugs have been recommended for cancer patients.
Such treatments lengthened the life or may permanently cure, but most treatments, experiences side effects such as miserable pain, blood clots fatigue, and infection. Cyclophosphamide CP is a nitrogen mustard alkylating agent[ 6 ] used in various types of cancer chemotherapy, but the International Agency for Research Centre has identified it as a carcinogen for both animals and humans.
MECC has high phenol content,[ 22 ] it is also a good source of antioxidant and antimutagenic activity.
The crude extract was dried in a water bath and kept for further uses.
Qualitative preliminary phytochemical screening was performed following. All the drugs and chemicals used in this experiment were procured from Segma sales, Silchar, Assam, India, which were kindly provided by Himedia, India. The assay was performed following[ 26 ] with slight modifications. Similarly, control was also read in the same wavelength by adding together of 0. With the same procedure measurement of the gallic acid standard was also recorded.
Curcuma caesia – Wikipedia
Percentage of inhibition was calculated using the formula given below:. Where Ab is the absorption of the control and Aa is the absorption of the extract sample. It was performed following[ 27 ] with slight modifications. The standard taken was gallic acid. The inhibition mixture was calculated using the formula:. Where, Ab is the absorption of the control and Aa is the absorption of the extract sample.
The animals were kindly procured from the Pasteur Institute, Shillong, India and were acclimatized for 15 days. The study was conducted on 25—30 g body weight male Swiss albino mice. They were maintained under controlled conditions of temperature and light 12 h light: They were provided standard mice feed. The experimental animals were divided into eight groups each containing five mice designated as follows:. After 7 days of the experimental period, the animals were sacrificed, and parameters described below were studied.
The blood sample was collected from the heart and kept it undisturbed for 2 h. Besides, the removed kidney and liver were washed with phosphate buffer saline and blotted with filter paper and kept in the deep freezer for further analysis. Mice bone curfuma micronucleus test was carried out according to. The cell suspensions were centrifuged at 10, rpm for 10 min and supernatant was removed. The pellet was resuspended in FBS before being used for preparing slides.
The air-dried slides were stained with May Grunwald stain and Geimsa cucuma.
Curcumma polychromatic erythrocytes PCEs were scored for each group of animals to determine the frequency of micronucleated polychromatic erythrocytes. All the slides were coded and scored by the same observer. The percentage reduction in the frequency of micronuclei was calculated using the formula given by[ 29 ]:.
Standard calibration curve for serum glutamic oxaloacetic transaminase and serum glutamic pyruvic transaminase. The assay was performed following[ 31 ] with slight modifications. The reaction mixtures were cooled and then centrifuged it.
Absorbance was recorded against blank. The percentage inhibition was calculated using the formula given below:. Glutathione reduced GSH was estimated following[ 32 ] with slight modifications.
Glutathione reductase GR was assayed in the liver and kidney following. The reaction mixture was incubated for 3 min, followed by the addition of 0. The absorbance was read at nm in spectrophotometer for every 15 s at czesia interval of 2—3 min. Protein curxuma was estimated following. The reaction mixtures contain 0. It was mixed through the vortex and incubated for 20 min. After 30 min of incubation cwesia was read at nm against the reagent blank. The amount of protein was estimated from the standard calibration curve obtained using bovine serum albumin [ Figure 2 ].
Preliminary phytochemical screening reveals the presence of alkaloids, carbohydrates, reducing sugars, flavonoids, terpenes, steroids, and tannins in the methanolic extract of C. Preliminary phytochemical screening of methanolic extract of Curcuma caesia Roxb. On the other hand, the highest inhibition Superoxide caesa scavenging ability of standard and methanolic extract of Curcuma caesia Roxb.
An increase in the number of micronuclei was observed when treated with CP only as shown in Table 4. The reduction percentage of micronuclei was There was no sign of toxicity in the treatment with extracts only since the values were almost near to normal groups, and they are not significantly different from the NC groups [ Table 4 ].
But their amount was found to be reduced with the administration of the extract at different concentrations. Medicinal plants and their derivatives have been used as an alternative to synthetic medicines in many countries. Medicinal plants play an important role in two-sided approach: One, plant-derived compounds are complex in nature which are difficult to synthesize in the laboratory and are helpful in the prevention of onset of cancer by its antioxidant activity and stimulation of the immune system,[ 35 ] second, plant-derived compounds are used for prevention and decreasing side effects of conventional cancer treatments.
The process of respiration, cell-mediated immune functions, and other process utilizing oxygen produces free radicals as an end product, continuously in the living body. CP under metabolic activation by cyt p produces metabolic products such as, hydroxycyclophosphamide which is used for chemoprevention[ 44 ] and acrolein ROS that cross-links DNA, also decrease the antioxidant activity. Peroxidation of lipids produces an end product, malondialdehyde MDA which disrupts the cell membrane, thereby increasing permeability to ions.
Determination of GSH is regarded as one of the most important factor to show the amount of antioxidant reserve in the organism. Our present work demonstrated that methaolic extract of rhizome of C. It is shown for the first time that MECC has protective effect against genotoxicity induced by CP in bone marrow cells as well as protects against toxicity induced in the liver and kidney by CP. Heisanam Pushparani DeviShe is a Research Scholar in the Department of Biotechnology, Assam University Silchar and her interest is on medicinal plant studies like antioxidant activity, antimutagenic activity, antigenotoxic activity etc.
Pranab Behari MazumderProfessor Pranab Behari Mazumder is a faculty of Department of Biotechnology, assam University, Silchar and his interest is on medicinal plant studies as well microbial related studies. Currently many of the Researchers is working under his guidance and many of them have awarded Ph.
D degree under his Guidence. National Center for Biotechnology InformationU. Journal List Pharmacognosy Res v. Author information Copyright and License information Disclaimer. This article has been cited by other articles in PMC.
Curcuma caesia – Black Turmeric
SUMMARY Cancer is characterized by uncontrolled growth of cells and much research has been done for the past several years from various disciplines for the treatment of cancer but till now no therapy has been discovered. Treatment of cancer with chemotherapeutic drugs has been suggested to prevent cancer cells however they are often limited with their toxicity to normal cells. Therefore it has been suggested that the supplementation of medicinal plants which are rich source of antioxidants can decrease the toxic effect caused by chemotherapeutic drugs.
Curcuma caesia Roxb is a medicinal plant which has high antioxidant activity, as per present study, methanolic extract of Curcuma caesia Roxb prevents the toxicity caused by cyclophosphosphamide chemotherapeutic drug in bone marrow cells by reducing the micronuclei formation; it also prevents the hepatotoxicity and nephrotoxicity caused by cyclophosphamide, so it can be used as a supplement in cancer treatment with cyclophosphamide.
Preliminary phytochemical screening Qualitative preliminary phytochemical screening was performed following. Drugs and chemicals All the drugs and chemicals used in this experiment were procured from Segma sales, Silchar, Assam, India, which were kindly provided by Himedia, India. Percentage of inhibition was calculated using the formula given below: Superoxide dismutase assay It was performed following[ 27 ] with slight modifications. The inhibition mixture was calculated using the formula: Experimental design The animals were kindly procured from the Pasteur Institute, Shillong, India and were acclimatized for 15 days.
The experimental animals were divided into eight groups each containing five mice designated as follows: Each animal received distilled water Group 2: Serum sample collection The blood sample was collected from the heart and kept it undisturbed for 2 h. Micronucleus assay Mice bone marrow micronucleus test was carried out according to.
The percentage reduction in the frequency of micronuclei was calculated using the formula given by[ 29 ]: Open in a separate window. Quantitative assay for lipid peroxidation The assay was performed following[ 31 ] with slight modifications. The percentage inhibition was calculated using the formula given below: